ColExM
Collagen expansion microscopy (ColExM) reveals nanoscale structures in collagen abundant tissues such as cornea, skin and tumor.
Figure 3. ColExM reveals super-resolution collagen fiber morphology with immunostaining. a) SHG images of nuclei (green) and collagen fibril/fiber
structures (magenta) in the footpad skin before and after expansion by ProK and collagenase digestion. The yellow and white arrowheads indicate the hair
follicles and hair shafts, respectively. b) Pre-ExM and post-ExM images of collagen I in the footpad skin. The yellow and white arrows indicate the thin and
thick collagen fibers, respectively. Scale bar unit: μm. Scale bars are not adjusted for the expansion factor.
Figure 6. Conformational change of tight junctions of corneal endothelial cells after EGTA treatment. a) Expression of Zo1 (green), F-actin (magenta),
and nuclei (gray) in the corneal endothelium before and after expansion without EGTA treatment and following EGTA treatment for 30 min, 1 h, and 2 h.
Colocalization of Zo1 and F-actin is shown in white in the merged images (yellow arrows). Scale bar unit: μm. Scale bars are not adjusted for the
expansion factor.